Protocols
Please note, in most cases these protocols have been optimised for certain conditions and are meant as a guide only, you may need to alter some parameters to suit your own experiment.
If you have a protocol you'd like to share with other users by adding it to this collection, please send it to detmold.staff@gmail.com.
| Cell sorting | |
| Sample prep guide.pdf | Download |
| 9 sorting protips (cytomad) | Link |
| Experimental design | |
| Controls for flow cytometry.pdf | Download |
| Virtue of a viability dye (cytomad) | Link |
| Titrating antibodies.pdf | Download |
| Cytospins.pdf | Download |
| 96-well plate staining tips.pdf | Download |
| Actin polymerisation.pdf | Download |
| Time parameter: post-aquisition data cleanup (cytomad) | Link |
| Cell Cycle / Viability | |
| Cell cycle info sheet | Download |
| Viability dye info sheet | Download |
| Proliferation assays | |
| CFSE staining.pdf | Download |
| Detection of in vivo proliferation by BrdU labelling.pdf | Download |
| Immunophenotyping assays | |
| Labelling human cells (direct).pdf | Download |
| Labelling human cells (indirect).pdf | Download |
| Intracellular cytokine staining (human).pdf | Download |
| Intracellular staining tips.pdf | Download |
| Labelling murine cells (direct).pdf | Download |
| Labelling murine cells (indirect).pdf | Download |
| Polyclonal staining (murine).pdf | Download |
| Reagent recipes & preparation | |
| Dialysis of antibodies.pdf | Download |
| IgG purification using Protein A or G columns.pdf | Download |
| Biotin labelling of antibodies.pdf | Download |
| FITC labelling of antibodies.pdf | Download |
| Murine lysis buffer.pdf | Download |
| Paraformaldehyde | Download |
| Wash solutions | Download |
Sorting Cheatsheet
